Metal free, 'click and click-click'' conjugation of ribonucleosides and 2'-OMe oligoribonucleotides on the solid phase

摘要

A fast and practical metal free conjugation of ribonucleosides and 2'-OMe 4-mer oligoribonucleotides has been accomplished by a nitrile oxide alkyne click cycloaddition reaction on the solid-phase, the methodology is suited to modification at either, or both, the 3'- or the 5'-terminus of the oligoribonucleotide substrate.